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Jeannie Peck and Donna Potter with Bill Treadway in 2014

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​WILLIAM TREADWAY TAUGHT AT SHS FROM 1960-1984

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Wastewater-based Tracing of Doping Use

The prevalence of doping in sport and in the community at large continues to be a 🛡 concern in many societies.

Numerous test protocols are in place to test individuals but the increased sensitivity of modern analytical techniques 🛡 and the purity of the ultrapure water available to use in testing have opened the scope for screening communal wastewater 🛡 for drugs and their metabolites.

Causanilles et al (1) have successfully developed and tested a procedure for 15 substances from the 🛡 groups of anabolic steroids (used to promote muscle growth), weight loss products (used to increase metabolism by burning fat) and 🛡 masking agents in wastewater using solid phase extraction to increase sensitivity and reduce matrix effects followed by liquid-chromatography coupled to 🛡 high resolution mass spectrometry.

50 ml of sample were spiked with an isotope-labeled internal standard, filtered, and acidified before concentration on 🛡 a mix-mode cationic polymer-based cartridge.

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If you bet on 0 or 00 on an American wheel, and your bet hits, you'll get a payout of 35 to 1. If you place a "row" bet (betting on both 0 and 00), and your bet hits, you'll get a payout of 17 to 1.

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The 0  (single zero) and the 00  (double zero) are the green slots on the roulette wheel. These pockets could receive a 35:1 payout if hit straight up. If there's a split, the payout could be 17:1. This type of bet is somewhat risky, but the payouts could be worth it.

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